flotillin (Novus Biologicals)
Structured Review

Flotillin, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flotillin/Flotillin-1+Antibody/pmc13088877-126-21-28
Average 92 stars, based on 8 article reviews
Images
1) Product Images from "Dysregulation of Extracellular Vesicle Concentration, MicroRNAs, and Surface Proteins in Patients With Niemann‐Pick Disease Type C"
Article Title: Dysregulation of Extracellular Vesicle Concentration, MicroRNAs, and Surface Proteins in Patients With Niemann‐Pick Disease Type C
Journal: Journal of Extracellular Biology
doi: 10.1002/jex2.70136
Figure Legend Snippet: SEC enriches for EVs from fibroblast conditioned media . ( A ) EV enrichment method for fibroblast conditioned media. ( B, C ) Immunoblots of individual ( B ) or pooled ( C ) SEC fractions from CTL fibroblast media show enrichment for EV proteins Flotillin‐1, CD63, and CD81, without detectable levels of other proteins (total protein stain) or cell lysate protein GM130. ( D ) MRPS histogram shows the distribution of particles in pooled EV fractions isolated from CTL fibroblast media. Mean particle count, size, and coefficient of variation are listed.
Techniques Used: Western Blot, Staining, Isolation
Figure Legend Snippet: SEC enriches for EVs from human CSF . ( A ) EV enrichment method for CSF. ( B, C ) Immunoblots of individual ( B ) or pooled ( C ) SEC fractions from CTL CSF show enrichment for EV proteins Flotillin‐1 and CD81 without detectable levels of bulk CSF proteins (total protein stain, albumin, ApoA1). ( D ) MRPS histogram shows the distribution of particles in pooled EV fractions from CTL CSF. Mean particle count, size, and coefficient of variation are listed. ( E ) TEM of representative NPC CSF EV sample. Black arrows indicate particles of ∼100 nm; white arrows indicate particles of ∼50 nm.
Techniques Used: Western Blot, Staining
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Western Blot:Article Title: TOM1 Regulates Neuronal Accumulation of Amyloid-β Oligomers by FcγRIIb2 Variant in Alzheimer's Disease Article Snippet: .. Western blotting and immunoprecipitation The following antibodies were used for Western blotting and immunoprecipitation: Aβ (4G8 and 6E10, both Covance); BiP, Calnexin, CHDH, GFP, mouse FcγRIIb, TOM1, Ubiquitin, (Santa Cruz); Article Title: TOM1 Regulates Neuronal Accumulation of Amyloid-β Oligomers by FcγRIIb2 Variant in Alzheimer's Disease Article Snippet: .. The following antibodies were used for Western blotting and immunoprecipitation: Aβ (4G8 and 6E10, both Covance); BiP, Calnexin, CHDH, GFP, mouse FcγRIIb, TOM1, Ubiquitin, (Santa Cruz); Article Title: Differential Effects of APOE Genotype on MicroRNA Cargo of Cerebrospinal Fluid Extracellular Vesicles in Females With Alzheimer's Disease Compared to Males. Article Snippet: Membranes were developed using the West Dura, Pico, or Femto chemiluminescence kits (Thermo Fisher Scientific) and imaged using the ChemiDoc Touch imaging system (Bio-Rad, Hercules, CA). .. The following antibodies were used for immunoblotting: albumin 1:1,000 (#4929, Cell Signaling Technology, Danvers, MA), APOA1 (12C8) 1:200 (sc-080551, Santa Cruz Biotechnology, Dallas, TX), APOE 1:2000 (50A-G1A, Academy Bio-medical Company, Inc., Houston, TX), AnnV 1: 5,000 (GTX103250, GeneTex, Irvine, CA), CD9 (C-4) 1:200 (sc13118, Santa Cruz Biotechnology), CD11b 1:1,000 (ab133357, Abcam, Cambridge, United Kingdom), CD63 1:1,000 (ab134045, Abcam), CD81 (B-11) 1:100 (sc-166029, Santa Cruz Biotechnology), Immunoprecipitation:Article Title: TOM1 Regulates Neuronal Accumulation of Amyloid-β Oligomers by FcγRIIb2 Variant in Alzheimer's Disease Article Snippet: .. Western blotting and immunoprecipitation The following antibodies were used for Western blotting and immunoprecipitation: Aβ (4G8 and 6E10, both Covance); BiP, Calnexin, CHDH, GFP, mouse FcγRIIb, TOM1, Ubiquitin, (Santa Cruz); Article Title: TOM1 Regulates Neuronal Accumulation of Amyloid-β Oligomers by FcγRIIb2 Variant in Alzheimer's Disease Article Snippet: .. The following antibodies were used for Western blotting and immunoprecipitation: Aβ (4G8 and 6E10, both Covance); BiP, Calnexin, CHDH, GFP, mouse FcγRIIb, TOM1, Ubiquitin, (Santa Cruz); Ubiquitin Proteomics:Article Title: TOM1 Regulates Neuronal Accumulation of Amyloid-β Oligomers by FcγRIIb2 Variant in Alzheimer's Disease Article Snippet: .. Western blotting and immunoprecipitation The following antibodies were used for Western blotting and immunoprecipitation: Aβ (4G8 and 6E10, both Covance); BiP, Calnexin, CHDH, GFP, mouse FcγRIIb, TOM1, Ubiquitin, (Santa Cruz); Article Title: TOM1 Regulates Neuronal Accumulation of Amyloid-β Oligomers by FcγRIIb2 Variant in Alzheimer's Disease Article Snippet: .. The following antibodies were used for Western blotting and immunoprecipitation: Aβ (4G8 and 6E10, both Covance); BiP, Calnexin, CHDH, GFP, mouse FcγRIIb, TOM1, Ubiquitin, (Santa Cruz); |