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flotillin  (Novus Biologicals)


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    Structured Review

    Novus Biologicals flotillin
    SEC enriches for EVs from fibroblast conditioned media . ( A ) EV enrichment method for fibroblast conditioned media. ( B, C ) Immunoblots of individual ( B ) or pooled ( C ) SEC fractions from CTL fibroblast media show enrichment for EV proteins <t>Flotillin‐1,</t> CD63, and CD81, without detectable levels of other proteins (total protein stain) or cell lysate protein GM130. ( D ) MRPS histogram shows the distribution of particles in pooled EV fractions isolated from CTL fibroblast media. Mean particle count, size, and coefficient of variation are listed.
    Flotillin, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flotillin/Flotillin-1+Antibody/pmc13088877-126-21-28
    Average 92 stars, based on 8 article reviews
    flotillin - by Bioz Stars, 2026-09
    92/100 stars

    Images

    1) Product Images from "Dysregulation of Extracellular Vesicle Concentration, MicroRNAs, and Surface Proteins in Patients With Niemann‐Pick Disease Type C"

    Article Title: Dysregulation of Extracellular Vesicle Concentration, MicroRNAs, and Surface Proteins in Patients With Niemann‐Pick Disease Type C

    Journal: Journal of Extracellular Biology

    doi: 10.1002/jex2.70136

    SEC enriches for EVs from fibroblast conditioned media . ( A ) EV enrichment method for fibroblast conditioned media. ( B, C ) Immunoblots of individual ( B ) or pooled ( C ) SEC fractions from CTL fibroblast media show enrichment for EV proteins Flotillin‐1, CD63, and CD81, without detectable levels of other proteins (total protein stain) or cell lysate protein GM130. ( D ) MRPS histogram shows the distribution of particles in pooled EV fractions isolated from CTL fibroblast media. Mean particle count, size, and coefficient of variation are listed.
    Figure Legend Snippet: SEC enriches for EVs from fibroblast conditioned media . ( A ) EV enrichment method for fibroblast conditioned media. ( B, C ) Immunoblots of individual ( B ) or pooled ( C ) SEC fractions from CTL fibroblast media show enrichment for EV proteins Flotillin‐1, CD63, and CD81, without detectable levels of other proteins (total protein stain) or cell lysate protein GM130. ( D ) MRPS histogram shows the distribution of particles in pooled EV fractions isolated from CTL fibroblast media. Mean particle count, size, and coefficient of variation are listed.

    Techniques Used: Western Blot, Staining, Isolation

    SEC enriches for EVs from human CSF . ( A ) EV enrichment method for CSF. ( B, C ) Immunoblots of individual ( B ) or pooled ( C ) SEC fractions from CTL CSF show enrichment for EV proteins Flotillin‐1 and CD81 without detectable levels of bulk CSF proteins (total protein stain, albumin, ApoA1). ( D ) MRPS histogram shows the distribution of particles in pooled EV fractions from CTL CSF. Mean particle count, size, and coefficient of variation are listed. ( E ) TEM of representative NPC CSF EV sample. Black arrows indicate particles of ∼100 nm; white arrows indicate particles of ∼50 nm.
    Figure Legend Snippet: SEC enriches for EVs from human CSF . ( A ) EV enrichment method for CSF. ( B, C ) Immunoblots of individual ( B ) or pooled ( C ) SEC fractions from CTL CSF show enrichment for EV proteins Flotillin‐1 and CD81 without detectable levels of bulk CSF proteins (total protein stain, albumin, ApoA1). ( D ) MRPS histogram shows the distribution of particles in pooled EV fractions from CTL CSF. Mean particle count, size, and coefficient of variation are listed. ( E ) TEM of representative NPC CSF EV sample. Black arrows indicate particles of ∼100 nm; white arrows indicate particles of ∼50 nm.

    Techniques Used: Western Blot, Staining

    Related Articles

    Western Blot:

    Article Title: TOM1 Regulates Neuronal Accumulation of Amyloid-β Oligomers by FcγRIIb2 Variant in Alzheimer's Disease
    Article Snippet: .. Western blotting and immunoprecipitation The following antibodies were used for Western blotting and immunoprecipitation: Aβ (4G8 and 6E10, both Covance); BiP, Calnexin, CHDH, GFP, mouse FcγRIIb, TOM1, Ubiquitin, (Santa Cruz); Flotillin, GM130, TIM23, CD49b (BD biosciences); human FcγRIIb, LC3 (Novus); α-tubulin, β-actin, APP, FLAG (Sigma Aldrich); PHF1 (Courtesy of Dr. Peter Davies, Albert Einstein College of Medicine). ..

    Article Title: TOM1 Regulates Neuronal Accumulation of Amyloid-β Oligomers by FcγRIIb2 Variant in Alzheimer's Disease
    Article Snippet: .. The following antibodies were used for Western blotting and immunoprecipitation: Aβ (4G8 and 6E10, both Covance); BiP, Calnexin, CHDH, GFP, mouse FcγRIIb, TOM1, Ubiquitin, (Santa Cruz); Flotillin, GM130, TIM23, CD49b (BD biosciences); human FcγRIIb, LC3 (Novus); α-tubulin, β-actin, APP, FLAG (Sigma Aldrich); PHF1 (Courtesy of Dr. Peter Davies, Albert Einstein College of Medicine). ..

    Article Title: Differential Effects of APOE Genotype on MicroRNA Cargo of Cerebrospinal Fluid Extracellular Vesicles in Females With Alzheimer's Disease Compared to Males.
    Article Snippet: Membranes were developed using the West Dura, Pico, or Femto chemiluminescence kits (Thermo Fisher Scientific) and imaged using the ChemiDoc Touch imaging system (Bio-Rad, Hercules, CA). .. The following antibodies were used for immunoblotting: albumin 1:1,000 (#4929, Cell Signaling Technology, Danvers, MA), APOA1 (12C8) 1:200 (sc-080551, Santa Cruz Biotechnology, Dallas, TX), APOE 1:2000 (50A-G1A, Academy Bio-medical Company, Inc., Houston, TX), AnnV 1: 5,000 (GTX103250, GeneTex, Irvine, CA), CD9 (C-4) 1:200 (sc13118, Santa Cruz Biotechnology), CD11b 1:1,000 (ab133357, Abcam, Cambridge, United Kingdom), CD63 1:1,000 (ab134045, Abcam), CD81 (B-11) 1:100 (sc-166029, Santa Cruz Biotechnology), flotillin 1:10,000 (ab133497, Abcam), GLAST 1:500 (NB100-1869, Novus Biologicals, Littleton, CO), NCAM1 1:125 (NBP2-38452, Novus Biologicals), SYP 1:1,000 (#36406, Cell Signaling Technology), TMEM119 (#66948-1-Ig, Proteintech, Rosemont, IL), and TSG101 1:1,000 (ab125011, Abcam). .. Secondary antibodies were purchased from Jackson ImmunoResearch (West Grove, PA) and included horseradish peroxidase (HRP)–conjugated donkey anti-mouse (#715-035- 150), donkey anti-rabbit (#711-035-152), and donkey anti-goat (#705-035-003).

    Immunoprecipitation:

    Article Title: TOM1 Regulates Neuronal Accumulation of Amyloid-β Oligomers by FcγRIIb2 Variant in Alzheimer's Disease
    Article Snippet: .. Western blotting and immunoprecipitation The following antibodies were used for Western blotting and immunoprecipitation: Aβ (4G8 and 6E10, both Covance); BiP, Calnexin, CHDH, GFP, mouse FcγRIIb, TOM1, Ubiquitin, (Santa Cruz); Flotillin, GM130, TIM23, CD49b (BD biosciences); human FcγRIIb, LC3 (Novus); α-tubulin, β-actin, APP, FLAG (Sigma Aldrich); PHF1 (Courtesy of Dr. Peter Davies, Albert Einstein College of Medicine). ..

    Article Title: TOM1 Regulates Neuronal Accumulation of Amyloid-β Oligomers by FcγRIIb2 Variant in Alzheimer's Disease
    Article Snippet: .. The following antibodies were used for Western blotting and immunoprecipitation: Aβ (4G8 and 6E10, both Covance); BiP, Calnexin, CHDH, GFP, mouse FcγRIIb, TOM1, Ubiquitin, (Santa Cruz); Flotillin, GM130, TIM23, CD49b (BD biosciences); human FcγRIIb, LC3 (Novus); α-tubulin, β-actin, APP, FLAG (Sigma Aldrich); PHF1 (Courtesy of Dr. Peter Davies, Albert Einstein College of Medicine). ..

    Ubiquitin Proteomics:

    Article Title: TOM1 Regulates Neuronal Accumulation of Amyloid-β Oligomers by FcγRIIb2 Variant in Alzheimer's Disease
    Article Snippet: .. Western blotting and immunoprecipitation The following antibodies were used for Western blotting and immunoprecipitation: Aβ (4G8 and 6E10, both Covance); BiP, Calnexin, CHDH, GFP, mouse FcγRIIb, TOM1, Ubiquitin, (Santa Cruz); Flotillin, GM130, TIM23, CD49b (BD biosciences); human FcγRIIb, LC3 (Novus); α-tubulin, β-actin, APP, FLAG (Sigma Aldrich); PHF1 (Courtesy of Dr. Peter Davies, Albert Einstein College of Medicine). ..

    Article Title: TOM1 Regulates Neuronal Accumulation of Amyloid-β Oligomers by FcγRIIb2 Variant in Alzheimer's Disease
    Article Snippet: .. The following antibodies were used for Western blotting and immunoprecipitation: Aβ (4G8 and 6E10, both Covance); BiP, Calnexin, CHDH, GFP, mouse FcγRIIb, TOM1, Ubiquitin, (Santa Cruz); Flotillin, GM130, TIM23, CD49b (BD biosciences); human FcγRIIb, LC3 (Novus); α-tubulin, β-actin, APP, FLAG (Sigma Aldrich); PHF1 (Courtesy of Dr. Peter Davies, Albert Einstein College of Medicine). ..



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    Image Search Results


    SEC enriches for EVs from fibroblast conditioned media . ( A ) EV enrichment method for fibroblast conditioned media. ( B, C ) Immunoblots of individual ( B ) or pooled ( C ) SEC fractions from CTL fibroblast media show enrichment for EV proteins Flotillin‐1, CD63, and CD81, without detectable levels of other proteins (total protein stain) or cell lysate protein GM130. ( D ) MRPS histogram shows the distribution of particles in pooled EV fractions isolated from CTL fibroblast media. Mean particle count, size, and coefficient of variation are listed.

    Journal: Journal of Extracellular Biology

    Article Title: Dysregulation of Extracellular Vesicle Concentration, MicroRNAs, and Surface Proteins in Patients With Niemann‐Pick Disease Type C

    doi: 10.1002/jex2.70136

    Figure Lengend Snippet: SEC enriches for EVs from fibroblast conditioned media . ( A ) EV enrichment method for fibroblast conditioned media. ( B, C ) Immunoblots of individual ( B ) or pooled ( C ) SEC fractions from CTL fibroblast media show enrichment for EV proteins Flotillin‐1, CD63, and CD81, without detectable levels of other proteins (total protein stain) or cell lysate protein GM130. ( D ) MRPS histogram shows the distribution of particles in pooled EV fractions isolated from CTL fibroblast media. Mean particle count, size, and coefficient of variation are listed.

    Article Snippet: Primary antibodies included NPC1 (EPR5209) 1:1000 (134113, Abcam); CD63 (H5C6) 1:5,000 (556019, BD Biosciences); CD81 (B‐11) 1:100 (sc‐166029, Santa Cruz Biotechnology); Flotillin‐1 1:10,000 (ab133497, Abcam); GM130 1:1000 (53420SS, Novus Biologicals); and Lamp1 (D2D11) 1:1000 (9091S XP, Cell Signaling Technology).

    Techniques: Western Blot, Staining, Isolation

    SEC enriches for EVs from human CSF . ( A ) EV enrichment method for CSF. ( B, C ) Immunoblots of individual ( B ) or pooled ( C ) SEC fractions from CTL CSF show enrichment for EV proteins Flotillin‐1 and CD81 without detectable levels of bulk CSF proteins (total protein stain, albumin, ApoA1). ( D ) MRPS histogram shows the distribution of particles in pooled EV fractions from CTL CSF. Mean particle count, size, and coefficient of variation are listed. ( E ) TEM of representative NPC CSF EV sample. Black arrows indicate particles of ∼100 nm; white arrows indicate particles of ∼50 nm.

    Journal: Journal of Extracellular Biology

    Article Title: Dysregulation of Extracellular Vesicle Concentration, MicroRNAs, and Surface Proteins in Patients With Niemann‐Pick Disease Type C

    doi: 10.1002/jex2.70136

    Figure Lengend Snippet: SEC enriches for EVs from human CSF . ( A ) EV enrichment method for CSF. ( B, C ) Immunoblots of individual ( B ) or pooled ( C ) SEC fractions from CTL CSF show enrichment for EV proteins Flotillin‐1 and CD81 without detectable levels of bulk CSF proteins (total protein stain, albumin, ApoA1). ( D ) MRPS histogram shows the distribution of particles in pooled EV fractions from CTL CSF. Mean particle count, size, and coefficient of variation are listed. ( E ) TEM of representative NPC CSF EV sample. Black arrows indicate particles of ∼100 nm; white arrows indicate particles of ∼50 nm.

    Article Snippet: Primary antibodies included NPC1 (EPR5209) 1:1000 (134113, Abcam); CD63 (H5C6) 1:5,000 (556019, BD Biosciences); CD81 (B‐11) 1:100 (sc‐166029, Santa Cruz Biotechnology); Flotillin‐1 1:10,000 (ab133497, Abcam); GM130 1:1000 (53420SS, Novus Biologicals); and Lamp1 (D2D11) 1:1000 (9091S XP, Cell Signaling Technology).

    Techniques: Western Blot, Staining